Biochemistry: Enzymes
Chemistry
biochemistry enzymes
Sample Data
| Front | Why do enzymes provide an alternative energetically favourable reaction pathway? |
| Back | enzymes do this to speed up the reaction but they don't change the energy of the reactants and products. So the equilibruim doesn't change, but the rate at which it is reached changed. |
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| Front | What are allosteric enzymes and how are they regulated? |
| Back | - Allosteric enzymes change shape when bound to an effector - this affects the binding affinity at different sites. - Effectors are molecules that regulate allosteric enzymes by non-conalently binding them to to a site other than the active site.- Positive and negative effectors can affect affinity of enzyme for substrate (K0.5) and the max catalytic activity of enzyme (Vmax)positive = increase enzyme activitynegative = inhibit enzyme activity |
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| Front | What is the Lineweaver-Burk plot? |
| Back | When V0is plotted against [S], it is not always possible to determine when Vmax has been achieved due to gradual upward slope.Lineweaver-Burk Plot (double-reciprocal plot) can be used to calculate Kmand Vmax
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