BIOL 3110: molecular biology
Biology
flash cards for molecular biology
Sample Data
| Text | ChIP = {{c1::chromatine immunopercipitation }} |
| Back Extra |
| Front | what are some tricks for coloing |
| Back | 1. phosphotase treatment of vector
 removing the phosphate, the sticky ends are no longer compatible but there is still a nick on the other side b/c of the black OH ligating two single strands, suffiecint enough to stabilize until transfered until e.coli in e.coli, it will get repaired to fix the nick 2. blue-white screening using the LacZ gene, insert something between and will break the lacZ gene will get coloured by b-galatosidase can collet either white or blue percipitate from dish the white are good ones 3. a) fill in overhangs to make blunt ends --> blund ends will always be compatable with each other can only fill in 5' overhang need to use poymerase and nucleotides to fill in ( T4 polymerase)
 3. b) chew back overhangs to produce blunt ends when overhang is in the 3' strand enzyme such as klenow fragment which is 3' -> 5' exonuclease
 4. adding adaptors or linkers adding sticky ends to blunt ends adds linkers then cut them to get sticky ends used to add known dna to bits of dna to sequence genomes adaptors skip the cutting step and already have stickey ends, one side is sticky end, one side is blunt prevents multiple adaptors to ligate to each other
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| Front | describe the bromo-domain Brd4 |
| Back |
 important for trancription elongation recuits p-TEFb to give signle to rna polymerase > phosphorylates rna pol II > gets released for trancription JQ1 developed by scientist that blocks the bindng of Brd4 importance - context of cancer
 physical traslocation and fusion to the NUT gene (nuclear protien in the testies) on some cancer patients the translocation creates Barret protien which mistargets Brd4 patients with this tranlocation develop dangerous cancer mouse if you treate with JQ1 > repress tumor > cure doesnt bind to acetylated histones and does not generate oncogensis
 brd4 activates MYC MY is overexpressed in 70% of cancers -> main driver of oncogensis in mice, treate with Jq 1 those are the results useful in mice JQ1 is toxic in humans :( Jq1 male contripceptive
 feed male mice with Jq 1 > done make sperm remove Jq 1 > make sperm again |