Microbiology Practicals (2nd MBBS)
Biology
A deck containing microbiology slides for bacteria, fungi, and parasites, with relevant questions. Spotters are there for culture media, basic lab equipment, etc.
This resource is based on the 2nd MBBS course at Kasturba Medical College, Manipal (India)
Sample Data
| Front |
 1. ID the medium2. What kind of medium is this and how does it function3. What is the use4. Name two other solid media used for the cultivation of this bacterium5. Prophylatic vaccine |
| Back | 1. ID the mediumTellurite Blood Agar with Corynebacterium Diphtheriae colonies2. What kind of medium is this and how does it functionSelective media: C. diptheriae appear as black colonies due to a reduction of potassium tellurite to metallic tellurium3. What is the useCulturation of C. diptheriae; differentiate the 3 biotypes: gravis, intermedius, mitis4. Name two other solid media used for the cultivation of this bacteriumLoeffler's serum slope; Tinsdale agar5. Prophylatic vaccineDPT |
| Tags | Microbiology::Practical Microbiology::Spotters::Media |
| Front |
 1. ID the parasite. Common name?2. Based on reproduction and habitat, kind of parasite?3. Mode of transmission and infective stage4. Three important pathological effects caused by adult parasite and one effect of larva5. Drug of choice |
| Back | 1. ID the parasite. Common name?Ascaris Lumbricoides; Roundworm2. Based on reproduction and habitat, kind of parasite?Oviparous, small intestinal nematode3. Mode of transmission and infective stageIngestion of food, drink contaminated with embryonated eggsInfective stage: embryonated egg, rhabditiform larvae4. Three important pathological effects caused by adult parasite and one effect of larvaAdult: loss of appetite, intestinal obstruction, protein malnutritionLarvae: Loeffler's syndrome5. Drug of choiceMabendazole |
| Tags | Microbiology::Practical Microbiology::Spotters::Parasites |
| Front |
 1. ID2. Interpret the reaction3. Application of the test |
| Back | 1. IDSugar Media2. Interpret the reactionPink = positive = fermentation of sugar with acid --> change in pHGas formation in tube 3 --> fermantation with gases3. Application of the testID of bacteria based on carbohydrate fermentation pattern |
| Tags | Microbiology::Practical Microbiology::Spotters::Media |